Nucleic Acid Question and Answer Essay Sample free essay sample

A. DNA Extraction Virtual Lab [ 2 Markss ] Question 1 [ 1. 0 grade ] Isolate nucleated cells into eppendorf tubing ( right get downing measure ) . Add 500 ul 10 % SDS and 55 ul protease K ( 10 mg/ml stock ) . Incubate at 37?C with soft commixture or rocking. Add 1. 4 milliliter saturated NaCl solution ( about 6M ) . Spin eppendorf tubings at 10000 revolutions per minute in a extractor for 15 proceedingss. Shake the tubing smartly for 15 seconds to let protein to precipitate. Transfer the supernatant to another eppendorf tubing. go forthing behind the precipitated protein pellet Add precisely two volumes of 100 % isopropyl alcohol at room temperature. Spin eppendorf tubings at 2500 revolutions per minute in a extractor for 15 proceedingss. Invert the tubing several times until the DNA precipitate is seeable. Remove supernatant from tubing. go forthing behind the precipitated DNA pellet. Dissolve DNA pellet in little volume of TE ( Tris-EDTA ) buffer or H2O ( right stoping measure ) . Question 2 [ 1. 0 grade ] When TE buffer or H2O is added. the Deoxyribonucleic acid pellet is able to fade out. We will write a custom essay sample on Nucleic Acid Question and Answer Essay Sample or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page However. in the presence of ethyl alcohol. the Deoxyribonucleic acid will precipitate. This is because. unlike H2O. ethyl alcohol has a low insulator invariable as it is less polar. That means that Na+ and PO3- could interact with each other more easy and do the Deoxyribonucleic acid to go less hydrophilic and be precipitated alternatively. B. Gel Electrophoresis Virtual Lab [ 2 Markss ] Question 1 [ 1. 0 grade ] The pieces of Deoxyribonucleic acid have to travel through the gel. where there will be some opposition. Large DNA fragments will confront more trouble in making so as they can non steal through the holes easy. Higher concentration of the gel will do the hole size to diminish so the Deoxyribonucleic acid can non go through through it easy. It will be slowed down and travel an even shorter distance or non be able to go much at all. This could take to inaccurate or indecipherable consequences. Question 2 [ 1. 0 grade ] Dye is used so as to better observe and track the motion of the atoms during cataphoresis. This is because we can non see the DNA coloring material with bare oculus. The lading dye will assist weigh down the Deoxyribonucleic acid so it will drop the underside of the gel and non float. C. PCR Virtual Lab [ 2 Markss ] Question 1 [ 1. 0 grade ] Extracted Deoxyribonucleic acid: this is of import so that we can retroflex the Deoxyribonucleic acid more times. It is used as a t emplet. Primer 1: Primer attach to the sites on the DNA strands that will be amplified so that they can copy specific DNA sequences without aiming the incorrect site. So Primer 1 will attach to the first site ( the start ) . Primer 2: Primer 2 will attach to the 2nd site ( the terminal ) . Nucleotides: Forms the base that makes up the Deoxyribonucleic acid codification. DNA polymerase: Attaches the Deoxyribonucleic acid codification it reads to the fiting base to do multiple transcripts of the DNA. Question 2 [ 1. 0 grade ] EDTA acts as a chelating agent. It binds cations and prevents enzymes from adhering to the Deoxyribonucleic acid. With increased concentration of the EDTA. there may non be good reaction conditions for Deoxyribonucleic acid polymerases due to extra of cations. This means that the Deoxyribonucleic acid can sometimes non be identified decently. However. there will non be any other new DNA fragments added so no 1 will be wrongly convicted. Entire / Maximum Marks: / 6 Markss